Cryogenically structured gelatin-based hydrogel as a resorbable macroporous matrix for biomedical technologies

Objective : to investigate the biological properties of a matrix made cryogenically structured hydrogel in form macroporous gelatin sponge, as well possibility creating cell-engineered constructs (CECs) on its basis. Materials and methods . The main components were (type A) obtained from porcine skin collagen, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide, (EDC) urea (all Sigma-Aldrich, USA). Surface morphology was examined using scanning electron microscopy (SEM). degree swelling water samples determined by gravimetric method. Cytotoxicity studied NIH3T3, fibroblast cell line isolated mouse, human adipose-derived mesenchymal stem/stromal cells (hAMSCs) IncuCyte ZOOM (EssenBioscience, metabolic activity hAMSCs assessed PrestoBlue™ reagents (Invitrogen™, To create CECs, we used hAMSCs, hepatocellular carcinoma HepG2 or umbilical vein endothelial lines EA.hy926. Albumin content culture medium enzyme immunoassay. Ammonia metabolism rate after 90 minutes incubation with 1 mM ammonium chloride (Sigma-Aldrich, USA) diluted day 15 experiment. Results Obtaining scaffold sponge included freezing an aqueous solution gelatin+urea mixture, removal polycrystals frozen solvent lyophilization, extraction ethanol treatment cryostructurate EDC. Scanning identified three types pores carrier surface: large (109 ± 17 μm), (39 10 small (16 6 μm). 3.8 0.2 g H 2 O per dry polymer. part CEC found have ability support adhesion proliferation EA.hy926 for 28, 9 days, respectively. secretion ammonia when cultured detected. Conclusion use gelatin-based tissue engineering products is shown be promising liver construct case.